Phytophthora sojae TatD nuclease positively regulates sporulation and negatively regulates pathogenesis.
Identifieur interne : 000199 ( Main/Exploration ); précédent : 000198; suivant : 000200Phytophthora sojae TatD nuclease positively regulates sporulation and negatively regulates pathogenesis.
Auteurs : Linlin Chen ; Danyu Shen ; Nannan Sun ; Jing Xu ; Wen Wang ; Daolong DouSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2014.
Descripteurs français
- KwdFr :
- Apoptose (MeSH), Désoxyribonucléases (MeSH), Expression des gènes (MeSH), Feuilles de plante (MeSH), Gènes rapporteurs (MeSH), Interactions hôte-parasite (MeSH), Maladies des plantes (parasitologie), Organismes génétiquement modifiés (MeSH), Phylogenèse (MeSH), Phytophthora (cytologie), Phytophthora (enzymologie), Phytophthora (génétique), Phytophthora (pathogénicité), Plant (MeSH), Protéines de fusion recombinantes (MeSH), Régulation de l'expression des gènes fongiques (MeSH), Soja (parasitologie), Spécificité d'espèce (MeSH), Séquence d'acides aminés (MeSH), Virulence (MeSH).
- MESH :
- cytologie : Phytophthora.
- enzymologie : Phytophthora.
- génétique : Phytophthora.
- parasitologie : Maladies des plantes, Soja.
- pathogénicité : Phytophthora.
- Apoptose, Désoxyribonucléases, Expression des gènes, Feuilles de plante, Gènes rapporteurs, Interactions hôte-parasite, Organismes génétiquement modifiés, Phylogenèse, Plant, Protéines de fusion recombinantes, Régulation de l'expression des gènes fongiques, Spécificité d'espèce, Séquence d'acides aminés, Virulence.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Apoptosis (MeSH), Deoxyribonucleases (MeSH), Gene Expression (MeSH), Gene Expression Regulation, Fungal (MeSH), Genes, Reporter (MeSH), Host-Parasite Interactions (MeSH), Organisms, Genetically Modified (MeSH), Phylogeny (MeSH), Phytophthora (cytology), Phytophthora (enzymology), Phytophthora (genetics), Phytophthora (pathogenicity), Plant Diseases (parasitology), Plant Leaves (MeSH), Recombinant Fusion Proteins (MeSH), Seedlings (MeSH), Soybeans (parasitology), Species Specificity (MeSH), Virulence (MeSH).
- MESH :
- chemical : Deoxyribonucleases, Recombinant Fusion Proteins.
- cytology : Phytophthora.
- enzymology : Phytophthora.
- genetics : Phytophthora.
- parasitology : Plant Diseases, Soybeans.
- pathogenicity : Phytophthora.
- Amino Acid Sequence, Apoptosis, Gene Expression, Gene Expression Regulation, Fungal, Genes, Reporter, Host-Parasite Interactions, Organisms, Genetically Modified, Phylogeny, Plant Leaves, Seedlings, Species Specificity, Virulence.
Abstract
During pathogenic interactions, both the host and pathogen are exposed to conditions that induce programmed cell death (PCD). Certain aspects of PCD have been recently examined in eukaryotic microbes but not in oomycetes. Here, we identified conserved TatD proteins in Phytophthora sojae; the proteins are key components of DNA degradation in apoptosis. We selected PsTatD4 for further investigation because the enzyme is unique to the oomycete branch of the phylogenetic tree. The purified protein exhibited DNase activity in vitro. Its expression was upregulated in sporangia and later infective stages but downregulated in cysts and during early infection. Functional analysis revealed that the gene was required for sporulation and zoospore production, and the expression levels were associated with the numbers of hydrogen-peroxide-induced terminal dUTP nick end-labeling-positive cells. Furthermore, overexpression of PsTatD4 gene reduced the virulence in a susceptible soybean cultivar. Together, these data suggest that apoptosis may play different roles in the early and late infective stages of P. sojae, and that PsTatD4 is a key regulator of infection. The association of PsTatD4 and apoptosis will lay a foundation to understanding the basic biology of apoptosis and its roles in P. sojae disease cycle.
DOI: 10.1094/MPMI-05-14-0153-R
PubMed: 24940989
Affiliations:
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when="2014">2014</date><idno type="RBID">pubmed:24940989</idno><idno type="pmid">24940989</idno><idno type="doi">10.1094/MPMI-05-14-0153-R</idno><idno type="wicri:Area/Main/Corpus">000195</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000195</idno><idno type="wicri:Area/Main/Curation">000195</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000195</idno><idno type="wicri:Area/Main/Exploration">000195</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Phytophthora sojae TatD nuclease positively regulates sporulation and negatively regulates pathogenesis.</title><author><name sortKey="Chen, Linlin" sort="Chen, Linlin" uniqKey="Chen L" first="Linlin" last="Chen">Linlin Chen</name></author><author><name sortKey="Shen, Danyu" sort="Shen, Danyu" uniqKey="Shen D" first="Danyu" last="Shen">Danyu Shen</name></author><author><name sortKey="Sun, Nannan" sort="Sun, Nannan" uniqKey="Sun N" first="Nannan" last="Sun">Nannan Sun</name></author><author><name sortKey="Xu, Jing" sort="Xu, Jing" uniqKey="Xu J" first="Jing" last="Xu">Jing Xu</name></author><author><name sortKey="Wang, Wen" sort="Wang, Wen" uniqKey="Wang W" first="Wen" last="Wang">Wen Wang</name></author><author><name sortKey="Dou, Daolong" sort="Dou, Daolong" uniqKey="Dou D" first="Daolong" last="Dou">Daolong Dou</name></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Apoptosis (MeSH)</term><term>Deoxyribonucleases (MeSH)</term><term>Gene Expression (MeSH)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Genes, Reporter (MeSH)</term><term>Host-Parasite Interactions (MeSH)</term><term>Organisms, Genetically Modified (MeSH)</term><term>Phylogeny (MeSH)</term><term>Phytophthora (cytology)</term><term>Phytophthora (enzymology)</term><term>Phytophthora (genetics)</term><term>Phytophthora (pathogenicity)</term><term>Plant Diseases (parasitology)</term><term>Plant Leaves (MeSH)</term><term>Recombinant Fusion Proteins (MeSH)</term><term>Seedlings (MeSH)</term><term>Soybeans (parasitology)</term><term>Species Specificity (MeSH)</term><term>Virulence (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Apoptose (MeSH)</term><term>Désoxyribonucléases (MeSH)</term><term>Expression des gènes (MeSH)</term><term>Feuilles de plante (MeSH)</term><term>Gènes rapporteurs (MeSH)</term><term>Interactions hôte-parasite (MeSH)</term><term>Maladies des plantes (parasitologie)</term><term>Organismes génétiquement modifiés (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Phytophthora (cytologie)</term><term>Phytophthora (enzymologie)</term><term>Phytophthora (génétique)</term><term>Phytophthora (pathogénicité)</term><term>Plant (MeSH)</term><term>Protéines de fusion recombinantes (MeSH)</term><term>Régulation de l'expression des gènes fongiques (MeSH)</term><term>Soja (parasitologie)</term><term>Spécificité d'espèce (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Virulence (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Deoxyribonucleases</term><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Maladies des plantes</term><term>Soja</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Plant Diseases</term><term>Soybeans</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Apoptosis</term><term>Gene Expression</term><term>Gene Expression Regulation, Fungal</term><term>Genes, Reporter</term><term>Host-Parasite Interactions</term><term>Organisms, Genetically Modified</term><term>Phylogeny</term><term>Plant Leaves</term><term>Seedlings</term><term>Species Specificity</term><term>Virulence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Apoptose</term><term>Désoxyribonucléases</term><term>Expression des gènes</term><term>Feuilles de plante</term><term>Gènes rapporteurs</term><term>Interactions hôte-parasite</term><term>Organismes génétiquement modifiés</term><term>Phylogenèse</term><term>Plant</term><term>Protéines de fusion recombinantes</term><term>Régulation de l'expression des gènes fongiques</term><term>Spécificité d'espèce</term><term>Séquence d'acides aminés</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">During pathogenic interactions, both the host and pathogen are exposed to conditions that induce programmed cell death (PCD). Certain aspects of PCD have been recently examined in eukaryotic microbes but not in oomycetes. Here, we identified conserved TatD proteins in Phytophthora sojae; the proteins are key components of DNA degradation in apoptosis. We selected PsTatD4 for further investigation because the enzyme is unique to the oomycete branch of the phylogenetic tree. The purified protein exhibited DNase activity in vitro. Its expression was upregulated in sporangia and later infective stages but downregulated in cysts and during early infection. Functional analysis revealed that the gene was required for sporulation and zoospore production, and the expression levels were associated with the numbers of hydrogen-peroxide-induced terminal dUTP nick end-labeling-positive cells. Furthermore, overexpression of PsTatD4 gene reduced the virulence in a susceptible soybean cultivar. Together, these data suggest that apoptosis may play different roles in the early and late infective stages of P. sojae, and that PsTatD4 is a key regulator of infection. The association of PsTatD4 and apoptosis will lay a foundation to understanding the basic biology of apoptosis and its roles in P. sojae disease cycle. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24940989</PMID><DateCompleted><Year>2014</Year><Month>11</Month><Day>07</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>27</Volume><Issue>10</Issue><PubDate><Year>2014</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>Phytophthora sojae TatD nuclease positively regulates sporulation and negatively regulates pathogenesis.</ArticleTitle><Pagination><MedlinePgn>1070-80</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-05-14-0153-R</ELocationID><Abstract><AbstractText>During pathogenic interactions, both the host and pathogen are exposed to conditions that induce programmed cell death (PCD). Certain aspects of PCD have been recently examined in eukaryotic microbes but not in oomycetes. Here, we identified conserved TatD proteins in Phytophthora sojae; the proteins are key components of DNA degradation in apoptosis. We selected PsTatD4 for further investigation because the enzyme is unique to the oomycete branch of the phylogenetic tree. The purified protein exhibited DNase activity in vitro. Its expression was upregulated in sporangia and later infective stages but downregulated in cysts and during early infection. Functional analysis revealed that the gene was required for sporulation and zoospore production, and the expression levels were associated with the numbers of hydrogen-peroxide-induced terminal dUTP nick end-labeling-positive cells. Furthermore, overexpression of PsTatD4 gene reduced the virulence in a susceptible soybean cultivar. Together, these data suggest that apoptosis may play different roles in the early and late infective stages of P. sojae, and that PsTatD4 is a key regulator of infection. The association of PsTatD4 and apoptosis will lay a foundation to understanding the basic biology of apoptosis and its roles in P. sojae disease cycle. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Linlin</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Shen</LastName><ForeName>Danyu</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Nannan</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Jing</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Wen</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Dou</LastName><ForeName>Daolong</ForeName><Initials>D</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. 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MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D036226" MajorTopicYN="N">Seedlings</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013025" MajorTopicYN="N">Soybeans</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>6</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>6</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>11</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24940989</ArticleId><ArticleId IdType="doi">10.1094/MPMI-05-14-0153-R</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Chen, Linlin" sort="Chen, Linlin" uniqKey="Chen L" first="Linlin" last="Chen">Linlin Chen</name><name sortKey="Dou, Daolong" sort="Dou, Daolong" uniqKey="Dou D" first="Daolong" last="Dou">Daolong Dou</name><name sortKey="Shen, Danyu" sort="Shen, Danyu" uniqKey="Shen D" first="Danyu" last="Shen">Danyu Shen</name><name sortKey="Sun, Nannan" sort="Sun, Nannan" uniqKey="Sun N" first="Nannan" last="Sun">Nannan Sun</name><name sortKey="Wang, Wen" sort="Wang, Wen" uniqKey="Wang W" first="Wen" last="Wang">Wen Wang</name><name sortKey="Xu, Jing" sort="Xu, Jing" uniqKey="Xu J" first="Jing" last="Xu">Jing Xu</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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